From 2009.igem.org
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| - | <h3>Components of the secretion system</h3>
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| - | <h4>Kuba</h4>
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| - | <br />
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| - | <p>Task:</p>
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| - | <ul>
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| - | <li>Isolation and restriction analysis of the components of the hemolysin secretion system</li>
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| - | </ul>
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| - | <br />
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| - | <p>The process</p>
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| - | <ul><li><p>Plasmids pLG612-1 (containing hemolysin secretion signal) and pVDL9 (other components of the secretion system) were kindly provided by dr Guzman.</p>
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| - | <p>plasmid isolation was preformed with the use of "Gel-out" kit (A&A Biotechnology)</p>
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| - | <br>
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| - | <br>
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| - | <p>pLG612 was cut with EcoRI and HindIII</p>
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| - | </li>
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| - | </ul>
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| - | <ul>
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| - | <br>
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| - | <li>Electrophoretic separation on 1% agarose gel</li>
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| - | </ul>
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| - | ,ul><p>Results:</p>
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| - | <img src="https://2009.igem.org/Image:PLG(E%26H).jpg></ul>
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| - |
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| - | <ul>
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| - | <li>Gel (from left)</li>
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| - | </ul>
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| - |
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| - | <ol>
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| - | <li>plasmid uncut (colony no. 1)</li>
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| - | <li>plasmid cut (colony no. 1)</li>
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| - | <li>plasmid uncut (colony no. 2) (is</li>
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| - | <li>plasmid cut (colony no. 2)</li>
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| - | <li>plasmid uncut (colony no. 3)</li>
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| - | <li>plasmid cut (colony no. 3)</li>
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| - | <li>GeneRuler DNA Ladder Mix #SM0333 (Fermentas)</li>
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| - | </ol>
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| - | <br>
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| - | <br>
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| - |
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| - | <ul><p>pVDL9 was cut with NotI</p>
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| - | <br>
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| - | <li>Electrophoretic separation on 1% agarose gel
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| - | <p>Results:</p>
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| - | <img src="https://2009.igem.org/Image:PVDL9(N).jpg">
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| - |
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| - | <li>Gel (from left)</li>
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| - | <ol>
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| - | <li>GeneRuler DNA Ladder Mix #SM0333 (Fermentas)</li>
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| - | <li>plasmid uncut (colony no. 1)</li>
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| - | <li>plasmid cut (colony no. 1)</li>
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| - | </ol>
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| - | </ul>
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Revision as of 01:31, 24 June 2009
both plasmids were isolated correctly
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