Team:Warsaw/Calendar-Main/23 June 2009
From 2009.igem.org
Components of the secretion system
Kuba
Task:
- Isolation and restriction analysis of the components of the hemolysin secretion system
The process
Plasmids pLG612-1 (containing hemolysin secretion signal) and pVDL9 (other components of the secretion system) were kindly provided by dr Guzman.
plasmid isolation was preformed with the use of "Gel-out" kit (A&A Biotechnology)
pLG612 was cut with EcoRI and HindIII
- Electrophoretic separation on 1% agarose gel
- Gel (from left)
- plasmid uncut (colony no. 1)
- plasmid cut (colony no. 1)
- plasmid uncut (colony no. 2) (is
- plasmid cut (colony no. 2)
- plasmid uncut (colony no. 3)
- plasmid cut (colony no. 3)
- GeneRuler DNA Ladder Mix #SM0333 (Fermentas)
- Electrophoretic separation on 1% agarose gel
Results:
<img src="https://2009.igem.org/Image:PVDL9(N).jpg">
- Gel (from left)
- GeneRuler DNA Ladder Mix #SM0333 (Fermentas)
- plasmid uncut (colony no. 1)
- plasmid cut (colony no. 1)
both plasmids were isolated correctly
April M T W T F S S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 May M T W T F S S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 June M T W T F S S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 July M T W T F S S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 August M T W T F S S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 September M T W T F S S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 October M T W T F S S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Results:
<img src="https://2009.igem.org/Image:PLG(E%26H).jpg">
pVDL9 was cut with NotI