Team:Warsaw/Calendar-Main/18 August 2009

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(New page: {{WarNotebook}} <!-- do not edit above me! --> <html> <h3><div style="text-align: center;">Assembly of endosomal detection operon</div></h3> <h4>Marcin</h4> <br/> <p>Task 1:</p> <ul> <li>...)
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<p>Task 1:</p>
<p>Task 1:</p>
<ul>
<ul>
-
<li>Gel-outs of following constructs digested in 17.08.09:</li>
+
<li>Gel-outs of following constructs digested on 17.08.09:</li>
<ul><li>p53 CDS on <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> plasmid</li>
<ul><li>p53 CDS on <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> plasmid</li>
<li><a href="http://partsregistry.org/Part:BBa_R0080"><span style="color: black">BBa_R0080</a></span> on <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> plasmid</li>
<li><a href="http://partsregistry.org/Part:BBa_R0080"><span style="color: black">BBa_R0080</a></span> on <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> plasmid</li>
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<p>Methods:</p><ul>
<p>Methods:</p><ul>
<li>All samples were inactivated via heating in 80 &deg;C for 20 minutes
<li>All samples were inactivated via heating in 80 &deg;C for 20 minutes
-
<li>All gel-outs were perform using the EurX gel-out kit according to the manual</a></li></ul>
+
<li>All gel-outs were performed using the EurX gel-out kit according to the manual</a></li></ul>
<p>Results</p>
<p>Results</p>
-
<ul><li>All gel-outs (except C0040 with RBS) were successful. One isolation fail because of very low DNA concentration</ul></li></br>
+
<ul><li>All gel-outs (except C0040 with RBS) were successful. One isolation failed because of very low DNA concentration</ul></li></br>
<p>Task 2:</p>
<p>Task 2:</p>
<ul>
<ul>
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1 &mu;l T4 ligase (Fermentas)
1 &mu;l T4 ligase (Fermentas)
</pre>
</pre>
-
<li>Ligation was perform about 9 hours and it was subsequently thermally inactivated</li>
+
<li>Ligation was performed for about 9 hours and it was subsequently thermally inactivated</li>
</ul>
</ul>

Revision as of 11:47, 19 August 2009


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Assembly of endosomal detection operon

Marcin


Task 1:

Methods:

  • All samples were inactivated via heating in 80 °C for 20 minutes
  • All gel-outs were performed using the EurX gel-out kit according to the manual

Results

  • All gel-outs (except C0040 with RBS) were successful. One isolation failed because of very low DNA concentration

Task 2:

  • Digest pKSII plasmid with cro CDS

Methods:

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