Team:Warsaw/Calendar-Main/1 September 2009
From 2009.igem.org
Making of the plac-RBS-llo-intA part
Jarek
Tasks:
- Isolation of plasmid DNA from liquid cultures.
- Digestion of DNA samples with EcoRI and PstI endonucleases.
Cloning of the mgtc promoter into the pSB1A3 plasmid
Kamil
Tasks:
- Bacteria transformation
Methods:
- A fresh batch of chemocompetent bacteria was transformed with the ligation mix and incubated on agarose plates containing double dose of ampicilin
Cloning of the cro-box into the pSB1A3 plasmid
Kamil
Tasks:
- Bacteria transformation
Methods:
- A fresh batch of chemocompetent bacteria was transformed with the ligation mix and incubated on agarose plates containing double dose of ampicilin
Ania
Tasks:
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