Team:Warsaw/Calendar-Main/20 September 2009
From 2009.igem.org
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Latest revision as of 22:51, 21 October 2009
Assembly of endosomal detection operon
Marcin
Task 1: Prepare bacterial culture to isolate BBa_K177044 from pSB1A3.
Task 2: Another ligation of BBa_K177044
Methods:
- Ligation mixtures composition:
10 μl digested insert 8 μl digested vector 5 μl Tango buffer(Fermentas) 2 μl ATP 10 mM solution 4 μl MQ water 1 μl ligase T4 (Fermentas)
- Ligation was carried out about 20 hours and subsequently inactivated
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