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Team:Warsaw/Calendar-Main/23 June 2009
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Revision as of 01:10, 24 June 2009
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Components of the secretion system
Kuba
Task:
- Isolation and restriction analysis of the components of the hemolysin secretion system
The process
Plasmids pLG612-1 (containing hemolysin secretion signal) and pVDL9 (other components of the secretion system) were kindly provided by dr Guzman.
plasmid isolation was preformed with the use of "Gel-out" kit (A&A Biotechnology)
pLG612 was cut with EcoRI and HindIII
- Electrophoretic separation on 1% agarose gel
- Gel (from left)
- plasmid uncut (colony no. 1)
- plasmid cut (colony no. 1)
- plasmid uncut (colony no. 2) (is
- plasmid cut (colony no. 2)
- plasmid uncut (colony no. 3)
- plasmid cut (colony no. 3)
- GeneRuler DNA Ladder Mix #SM0333 (Fermentas)
- Electrophoretic separation on 1% agarose gel
- Gel (from left)
- GeneRuler DNA Ladder Mix #SM0333 (Fermentas)
- plasmid uncut (colony no. 1)
- plasmid cut (colony no. 1)
Results:
<img src="https://2009.igem.org/Image:PLG(E%26H).jpg"/>
pVDL9 was cut with NotI
</li> </ul>
Results:
<img src="https://2009.igem.org/Image:PVDL9(N).jpg"/> <var>
both plasmids were correctly isolated
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