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Team:Warsaw/Calendar-Main/28 May 2009
From 2009.igem.org
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description of the program is [https://2009.igem.org/Team:Warsaw/Calendar-Main/13_July_2009 here] | description of the program is [https://2009.igem.org/Team:Warsaw/Calendar-Main/13_July_2009 here] | ||
Revision as of 16:57, 14 July 2009
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digest of constructs pKS/hly
Marcin
- Task:
Restriction digest of isolated plasmids pKS/hly to confirm the correctness of the ligation Methods:
- Composition of reaction mixture:1 μl purified plasmid solution, 0.5 μl HindIII (Fermentas), 2 μl Buffer Red (Fermentas), 15.5 μl MQ water
- Digest:
Program:
description of the program is here
After digest samples were loaded into gel and electrophoretically separated
Electrophoresis conditions:
voltage - 100V
time: 40 minutes
Comment:
Sample 1 and 2 have expected resctricion pattern.
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