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Team:Warsaw/Calendar-Main/2 July 2009
From 2009.igem.org
(Difference between revisions)
(New page: {{WarNotebook}} <!-- do not edit above me! --> <html> <h3>cox sequence</h3> <h4>Seba</h4> <br /> <p>Tasks:</p> <ul> <li>Isolation pAcGFP1-Mito</li> <p>Methods:</p> <p>Isolation of plas...) |
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<html> | <html> | ||
| - | <h3> | + | <h3>Cloning of mitochondrial targeting signal</h3> |
<h4>Seba</h4> | <h4>Seba</h4> | ||
<br /> | <br /> | ||
<p>Tasks:</p> | <p>Tasks:</p> | ||
<ul> | <ul> | ||
| - | <li>Isolation pAcGFP1-Mito</li> | + | <li>Isolation of the pAcGFP1-Mito plasmid (containing mitochondrial targeting signal from subunit VII ot the human cytochrome C oxidase (Cox))</li> |
<p>Methods:</p> | <p>Methods:</p> | ||
| - | <p>Isolation of plasmid DNA from 1,5ml of overnight bacterial | + | <p>Isolation of plasmid DNA from 1,5ml of overnight bacterial culture with Plasmid Mini kit (A&A Biotechnology)</p> |
<p>Plasmid concentration in isolated sample - 50ng/ul</p> | <p>Plasmid concentration in isolated sample - 50ng/ul</p> | ||
Revision as of 21:49, 7 July 2009
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Cloning of mitochondrial targeting signal
Seba
Tasks:
- Isolation of the pAcGFP1-Mito plasmid (containing mitochondrial targeting signal from subunit VII ot the human cytochrome C oxidase (Cox))
Methods:
Isolation of plasmid DNA from 1,5ml of overnight bacterial culture with Plasmid Mini kit (A&A Biotechnology)
Plasmid concentration in isolated sample - 50ng/ul
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