"
Team:Warsaw/Calendar-Main/3 September 2009
From 2009.igem.org
(Difference between revisions)
(→Preparation of glycerol stocks) |
|||
| Line 23: | Line 23: | ||
*Preparation of glycerol stocks following the prodcedure described [https://2009.igem.org/Team:Warsaw/protocols#Preparation_of_glycerol_stocks <span style="color: blue;">here</span>] | *Preparation of glycerol stocks following the prodcedure described [https://2009.igem.org/Team:Warsaw/protocols#Preparation_of_glycerol_stocks <span style="color: blue;">here</span>] | ||
| - | |||
| - | + | pAraC + GFP | |
| - | plac + RBS + cI + | + | plac + RBS + cI + ter |
| - | plac + RBS + cI + RBS + GFP + | + | plac + RBS + cI + RBS + GFP + ter |
| + | J07037 | ||
| + | plac | ||
| + | |||
| + | pSB6A1 | ||
Revision as of 11:58, 8 September 2009
 |  |
 |
  |
 |
|
Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome
Jarek
Tasks:
- Digestion of PCR products, separetly with MboI and CaiI restriction endonucleases
- Electrophoretical segregation of digested samples in 1,5% agarose gel
- Preparation of another PCR reaction with L.monocytogenes genome DNA
Results:
- There were no visible products of digestion, even after long treatment with ethidium bromide. Propably the Clean-Up kit isn't working well. I'll try to acquire IntA gene with another PCR.
Preparation of glycerol stocks
Preparation of glycerol stocks
Monika
- Preparation of glycerol stocks following the prodcedure described here
pAraC + GFP
plac + RBS + cI + ter
plac + RBS + cI + RBS + GFP + ter
J07037
plac
pSB6A1
|
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
