Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome

Jarek

Tasks:

• Digestion of PCR products, separetly with MboI and CaiI restriction endonucleases
• Electrophoretical segregation of digested samples in 1,5% agarose gel
• Preparation of another PCR reaction with L.monocytogenes genome DNA

Results:

• There were no visible products of digestion, even after long treatment with ethidium bromide. Propably the Clean-Up kit isn't working well. I'll try to acquire IntA gene with another PCR.

Preparation of glycerol stocks

• Preparation of glycerol stocks following the prodcedure described here

plac + GFP

AraC + GFP

plac + RBS + cI + terminator

plac + RBS + cI + RBS + GFP + terminator