Team:Warsaw/Calendar-Main/4 September 2009
From 2009.igem.org
Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome
Jarek
Tasks:
- Electrophoretical segregation of PCR products from previous day in 0,8% agarosis gel
- Preparation of another PCR reaction with L.monocytogenes genome DNA
Results:
- There were no visible products on gel, it might happened that I accidently forget to add one of the substrates to reaction.
Assembly of endosomal detection operon
Marcin
Task 1:
- Transformation of chemocompetent E. coli TOP10 strain. Construct to transform:
Methods:
- Detailed protocol of transformation is described here
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