Team:Warsaw/Calendar-Main/5 July 2009
From 2009.igem.org
(Difference between revisions)
(New page: {{WarNotebook}} <!-- do not edit above me! --><html> <h3>Cloning of p53 coding sequence</h3> <h4>Marcin</h4> <br/> <p>Tasks:</P> <ul> <li>Transformation of chemocompetent E. coli strain DH...) |
|||
Line 17: | Line 17: | ||
<li>add 800 ul of SOB medium to the bacteria</li> | <li>add 800 ul of SOB medium to the bacteria</li> | ||
<li>incubation in 37°C - 1 h</li> | <li>incubation in 37°C - 1 h</li> | ||
- | <li>Plating on | + | <li>Plating on selective LB medium supplemented with Ampicilin, X-Gal and IPTG</li> |
</html> | </html> | ||
Revision as of 00:04, 9 July 2009
Cloning of p53 coding sequence
Marcin
Tasks:
- Transformation of chemocompetent E. coli strain DH5alpha
Methods:
|
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|