Team:Uppsala-Sweden/Notebook
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All primers we used during the project can be found here: [[Media:iGEMUppsala09_Primer.xls]]<br> | All primers we used during the project can be found here: [[Media:iGEMUppsala09_Primer.xls]]<br> | ||
Annealing temperatures etc. can be found in the notebook (see below). | Annealing temperatures etc. can be found in the notebook (see below). | ||
+ | |||
+ | ==Protocols== | ||
+ | |||
+ | ===PCR Setup=== | ||
+ | [[Media:PCR MM and Primers.xls]] | ||
+ | |||
+ | ===Transformation Protocol=== | ||
+ | [[Media:Transformation workorder.pdf]] | ||
+ | |||
+ | ===Growth media for <i>Z. mobilis</i>=== | ||
+ | [[Media:Fullmedium mobilis.xls]] | ||
+ | [[Media:Fullmedium mobilis plus Agar.xls]] | ||
+ | |||
+ | ===Growth medium for <i>Synechocystis sp PCC 6803</i>=== | ||
+ | [[Media:BG11.doc]] | ||
+ | |||
+ | ===Growth medium for <i>E. coli</i>=== | ||
+ | [[Media:LB.doc]] | ||
+ | |||
+ | ===Various=== | ||
+ | General Glycerol Stock Protocol: | ||
+ | [[Media:Glycerol Stocks.xls]] | ||
== '''Notebook''' == | == '''Notebook''' == |
Revision as of 17:07, 20 October 2009
Contents |
Oligonucleotides used by the Uppsala iGEM Team 2009
All primers we used during the project can be found here: Media:iGEMUppsala09_Primer.xls
Annealing temperatures etc. can be found in the notebook (see below).
Protocols
PCR Setup
Transformation Protocol
Media:Transformation workorder.pdf
Growth media for Z. mobilis
Media:Fullmedium mobilis.xls Media:Fullmedium mobilis plus Agar.xls
Growth medium for Synechocystis sp PCC 6803
Growth medium for E. coli
Various
General Glycerol Stock Protocol: Media:Glycerol Stocks.xls
Notebook
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