Team:Uppsala-Sweden/Notebook

From 2009.igem.org

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{{Uppsala-Sweden Template}}
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== '''Notebook''' ==
 
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==Notebook==
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{| align="center"
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=06}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=07}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=08}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=09}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=10}}
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==Oligonucleotides used by the Uppsala iGEM Team 2009==
==Oligonucleotides used by the Uppsala iGEM Team 2009==
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==Protocols==
==Protocols==
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===PCR Setup===
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====PCR Setup====
[[Media:PCR MM and Primers.xls]]
[[Media:PCR MM and Primers.xls]]
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===Transformation Protocol===
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====Transformation Protocol====
[[Media:Transformation workorder.pdf]]
[[Media:Transformation workorder.pdf]]
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===Growth media for <i>Z. mobilis</i>===
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====Growth media for <i>Z. mobilis</i>====
[[Media:Fullmedium mobilis.xls]]<br>
[[Media:Fullmedium mobilis.xls]]<br>
[[Media:Fullmedium mobilis plus Agar.xls]]
[[Media:Fullmedium mobilis plus Agar.xls]]
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===Growth medium for <i>Synechocystis sp PCC 6803</i>===
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====Growth medium for <i>Synechocystis sp PCC 6803</i>====
[[Media:BG11.pdf]]
[[Media:BG11.pdf]]
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===Growth medium for <i>E. coli</i>===
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====Growth medium for <i>E. coli</i>====
[[Media:LB.pdf]]
[[Media:LB.pdf]]
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===Various===
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====Various====
General Glycerol Stock Protocol:<br>
General Glycerol Stock Protocol:<br>
[[Media:Glycerol Stocks.xls]]
[[Media:Glycerol Stocks.xls]]
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=06}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=07}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=08}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=09}}
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{{Uppsala-Sweden_Footer}}
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Revision as of 17:34, 20 October 2009




Notebook

June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31


Oligonucleotides used by the Uppsala iGEM Team 2009

All primers we used during the project can be found here: Media:iGEMUppsala09_Primer.xls
Annealing temperatures etc. can be found in the notebook (see below).

Protocols

PCR Setup

Media:PCR MM and Primers.xls

Transformation Protocol

Media:Transformation workorder.pdf

Growth media for Z. mobilis

Media:Fullmedium mobilis.xls
Media:Fullmedium mobilis plus Agar.xls

Growth medium for Synechocystis sp PCC 6803

Media:BG11.pdf

Growth medium for E. coli

Media:LB.pdf

Various

General Glycerol Stock Protocol:
Media:Glycerol Stocks.xls






Bioneer Biolegio Clontech Uppsala Genome Center