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Team:Warsaw/TOC
From 2009.igem.org
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Cloning of hly gene into pKSII vector
PCR inv
EMSA
isolation of genomic DNA from S. typhimurium
PCR mgtc, pho
PCR pho, mgtc, inv
Testing of Biobrick parts obtained from the registry
Preparation of chemocompetent E.coli DH5α
Testing of BioBricks from 2009 distribution and pVDL9 plasmid
Team meeting
Making Llo fusion with secretion signal peptide
Cloning of mitochondrial targeting signal
Quality check (cro PCR product and pKS isolate)
Cloning of p53 coding sequence
Informal meeting concerning important issues of the Project
Miecznikowa team Aim: debug RFP-terminator ligation that did not work
Creating devices to test promoters in E. coli strains (devices BBa_K177024 and BBa_K177025)
Isolation of BioBricks from 2008 and 2009 Kit Plates
Construction of K177012 operon1_part2
Insertion of the pho gene into the pKSII plasmid
Evaluating the quality of bacterial medium
Franek lub Monika opisze minilizy dla Moniki
Assembly of endosomal detection operon
Testing different E. coli strains regarding lacI and AraC repressors
Assembly of invasion operon
Parts preparations
Construction of K177012 operon1_part2
PCR of Cox mitochondrial export sequence
Preparation of the electrocompetent bacteria
Cloning of the mgtc promoter into the pSB1A3 plasmid
Construction of RBS.3-listeriolysin
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