Team:Uppsala-Sweden/Notebook
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+ | ==Notebook== | ||
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+ | |align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=06}} | ||
+ | |align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=07}} | ||
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+ | |align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=09}} | ||
+ | |align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=10}} | ||
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==Oligonucleotides used by the Uppsala iGEM Team 2009== | ==Oligonucleotides used by the Uppsala iGEM Team 2009== | ||
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==Protocols== | ==Protocols== | ||
- | ===PCR Setup=== | + | ====PCR Setup==== |
[[Media:PCR MM and Primers.xls]] | [[Media:PCR MM and Primers.xls]] | ||
- | ===Transformation Protocol=== | + | ====Transformation Protocol==== |
[[Media:Transformation workorder.pdf]] | [[Media:Transformation workorder.pdf]] | ||
- | ===Growth media for <i>Z. mobilis</i>=== | + | ====Growth media for <i>Z. mobilis</i>==== |
[[Media:Fullmedium mobilis.xls]]<br> | [[Media:Fullmedium mobilis.xls]]<br> | ||
[[Media:Fullmedium mobilis plus Agar.xls]] | [[Media:Fullmedium mobilis plus Agar.xls]] | ||
- | ===Growth medium for <i>Synechocystis sp PCC 6803</i>=== | + | ====Growth medium for <i>Synechocystis sp PCC 6803</i>==== |
[[Media:BG11.pdf]] | [[Media:BG11.pdf]] | ||
- | ===Growth medium for <i>E. coli</i>=== | + | ====Growth medium for <i>E. coli</i>==== |
[[Media:LB.pdf]] | [[Media:LB.pdf]] | ||
- | ===Various=== | + | ====Various==== |
General Glycerol Stock Protocol:<br> | General Glycerol Stock Protocol:<br> | ||
[[Media:Glycerol Stocks.xls]] | [[Media:Glycerol Stocks.xls]] | ||
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Revision as of 17:34, 20 October 2009
Notebook
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Oligonucleotides used by the Uppsala iGEM Team 2009
All primers we used during the project can be found here: Media:iGEMUppsala09_Primer.xls
Annealing temperatures etc. can be found in the notebook (see below).
Protocols
PCR Setup
Transformation Protocol
Media:Transformation workorder.pdf
Growth media for Z. mobilis
Media:Fullmedium mobilis.xls
Media:Fullmedium mobilis plus Agar.xls
Growth medium for Synechocystis sp PCC 6803
Growth medium for E. coli
Various
General Glycerol Stock Protocol:
Media:Glycerol Stocks.xls