Team:Warsaw/Calendar-Main/22 August 2009
From 2009.igem.org
Assembly of endosomal detection operon
Marcin
Task 1:
- Electrophoresis of plasmid isolated and subsequently digested in: 21.08.09
Results:
All samples of cro CDS on pSB1A3 are without insert
Comment:
The most probable explanation of this situation is circularization of empty plasmid because I have forgotten to dephosphorylate vector using CIAP
Task 2:
- Prepare the bacterial cultures for isolation of following construct: BBa_C0040 with BBa_B0032 + BBa_R0080 on pSB1A3 plasmid
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