Wisconsin-Madison/12 July 2009
From 2009.igem.org
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July 12, 2009
Cut lac with E and Spec2 Buffer EcorI DIGEST
RFP and RBS Eco Xba1
PCR Clean UP
Spec Ligation
TRANSFORM Trip __> DH1 Anp test
Lac RFP (RBS ---dh10b
Lac 50
Rfp rbs 90 ug/ml
Speed Vac
Ligation 2.5 RFP 5.5 Lac 2 buffer 1 ligase
- added 100mL media A to 7/9 natural transformation test cultures and 100uL CM - NP