Wisconsin-Madison/12 June 2009
From 2009.igem.org
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June 12, 2009
Ex 11: Salt tolerance of E.coli DH10B:
Use plate reading machine to chart OD over time
ExA: 30uL of DH10B ecoli, 200uL of LB
OD taken every 30 min for 48 hours
Could not start today because Rebecca stole our Plate reader time slot
Ex 10: Made BG-11 plates
Other: Induced nud F
Freezer stock
Transformation of spinach cdna success.
- received the order of primers (diluted 10uL/nmol) - NP
- remade freezer stocks of pSB1A3, pRL1383A, and pMMBRraA - NP
- induced nudF cultures - NP
- did boiling lysis mini preparation of pRL1383A - NP