Wisconsin-Madison/3 September 2009
From 2009.igem.org
Calendar
|
|
|
|
|
|
|
September 3, 2009
miniprep digest and gel of potential clones
transformation of ProU/GsSDMT
nudF sequencing
.5 uL DNA (100 ng)
1 uL Big Dye
1.5uL Big Dye Buffer
.25uL Primer (fwd or rev)
5.75uL H2O
Sequencing clean-up using magnetic beads
Confirm sequencing results that nudF worked
Digestion confirmation of GSMT, photosystem promoter and pSB1A2