Wisconsin-Madison/7 July 2009

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July 7, 2009

Ex 10: Cyanobacteria

Medi Prep of mmbbraA


Ex 20:

G. Transformation (2/3 uL plasmid – have been getting low ms numbers)

1. NudF: Amp (5.50ms)

2. Choline: Amp (4.80ms)

3. Backbone P1: Amp (5.70ms)


37 C Water bath for 40 minutes

Pellet Samples and plate 50uL


Ex 14: and Ex 19:

Growth:

0707-plate..png

Inoculation of Tranformed Cells

M12 GFP: amp

gsSDMT: kanamycin


Ex 19: Parts from the Registry

I2.9B Lac+GFP

OD600

0.317 0.326

0.500 *Induced

Induced with 10uL of IPTG stock / 10mL culture

IPTG Stock is found in fridge in Brian’s Lab in “Antibiotics and Induction” Box

Results: For some reason cells died and formed chunks… ?

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