Wisconsin-Madison/24 August 2009
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Latest revision as of 01:31, 21 October 2009
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August 24, 2009
digest of possible pBAD24 clone with AflIII should give fragments of size 1500, 1680, 3470
2uL DNA
1uL AflIII
13uL Water
1.8uL NEB Buffer III
.18uL BSA
two hour digest
Inoculated possible ProU/RFP in salt solutions, plated on NaCl amp plate.