Wisconsin-Madison/3 August 2009
From 2009.igem.org
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===='''August 3, 2009'''==== | ===='''August 3, 2009'''==== | ||
* Received New MevT with Arabinose promoter | * Received New MevT with Arabinose promoter | ||
- | + | -Streaked Plate | |
* Inoculation: pSB1A3 | * Inoculation: pSB1A3 | ||
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* Plasmid Prep: | * Plasmid Prep: | ||
- | + | -Single Digestion (two cuts) – GOOD | |
* Cyanos: Sterile Filtration Flasks | * Cyanos: Sterile Filtration Flasks |
Revision as of 00:38, 21 October 2009
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August 3, 2009
- Received New MevT with Arabinose promoter
-Streaked Plate
- Inoculation: pSB1A3
- Received Taq sequencing – looks good
- Plasmid Prep:
-Single Digestion (two cuts) – GOOD
- Cyanos: Sterile Filtration Flasks
- Boiling Lysis of pmmBrRaA
- Transformation of PCC 7942 with pmmBrRaA
- 2nd Freezer Stock Test