Wisconsin-Madison/3 August 2009

From 2009.igem.org

(Difference between revisions)
(August 3, 2009)
(August 3, 2009)
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===='''August 3, 2009'''====
===='''August 3, 2009'''====
* Received New MevT with Arabinose promoter
* Received New MevT with Arabinose promoter
-
-Streaked Plate
+
-Streaked Plate
 
 
* Inoculation: pSB1A3
* Inoculation: pSB1A3
Line 51: Line 51:
 
 
* Plasmid Prep:
* Plasmid Prep:
-
 -Single Digestion (two cuts) – GOOD
+
-Single Digestion (two cuts) – GOOD
 
 
* Cyanos: Sterile Filtration Flasks
* Cyanos: Sterile Filtration Flasks

Revision as of 00:38, 21 October 2009

Calendar

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31

August 3, 2009

  • Received New MevT with Arabinose promoter

-Streaked Plate  

  • Inoculation: pSB1A3

                   

  • Received Taq sequencing – looks good

 

  • Plasmid Prep:

-Single Digestion (two cuts) – GOOD  

  • Cyanos: Sterile Filtration Flasks
  • Boiling Lysis of pmmBrRaA
  • Transformation of PCC 7942 with pmmBrRaA
  • 2nd Freezer Stock Test