Wisconsin-Madison/3 August 2009
From 2009.igem.org
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* Received New MevT with Arabinose promoter | * Received New MevT with Arabinose promoter | ||
-Streaked Plate | -Streaked Plate | ||
- | + | * Inoculation: pSB1A3 | |
- | * Inoculation: | + | |
- | + | ||
* Received Taq sequencing – looks good | * Received Taq sequencing – looks good | ||
- | |||
* Plasmid Prep: | * Plasmid Prep: | ||
-Single Digestion (two cuts) – GOOD | -Single Digestion (two cuts) – GOOD | ||
- | |||
* Cyanos: Sterile Filtration Flasks | * Cyanos: Sterile Filtration Flasks | ||
* Boiling Lysis of pmmBrRaA | * Boiling Lysis of pmmBrRaA | ||
* Transformation of PCC 7942 with pmmBrRaA | * Transformation of PCC 7942 with pmmBrRaA | ||
* 2nd Freezer Stock Test | * 2nd Freezer Stock Test |
Latest revision as of 00:39, 21 October 2009
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August 3, 2009
- Received New MevT with Arabinose promoter
-Streaked Plate
- Inoculation: pSB1A3
- Received Taq sequencing – looks good
- Plasmid Prep:
-Single Digestion (two cuts) – GOOD
- Cyanos: Sterile Filtration Flasks
- Boiling Lysis of pmmBrRaA
- Transformation of PCC 7942 with pmmBrRaA
- 2nd Freezer Stock Test