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Wisconsin-Madison/4 August 2009
From 2009.igem.org
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* Inoculated mevT, pBAD24, PSB1A3 | * Inoculated mevT, pBAD24, PSB1A3 | ||
| + | * prep of more PSB1A3 | ||
| + | * gel extraction of pBAD24, PSB1A3, GsSDMT, and PCR GsSDMT | ||
| + | * double digest of the above with appropriate enzymes | ||
| + | * ligation of the biobrick attempt | ||
| + | * cyano transformation attempt | ||
Latest revision as of 00:46, 21 October 2009
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August 4, 2009
Cyanos: Transformations à 30°C room with Parafilm 1. Low 1 – Culture started on 7/27/09 - 23 mg/ml 2. Low 2 - Culture started on 7/27/09 – 32 mg/ml 3. High 1 - Culture started on 7/20/09 – 23 mg/ml 4. High 2 - Culture started on 7/20/09 – 23 mg/ml
- Liquid cultures of High 1 and Low 1 were placed in 30°C Shaker, minimal indirect light
- Plated Cultures of High 1 and Low 1 were placed in minimal indirect light
C: Plating: 1% BG-11 – no resistance: 40mL C: Plating: 1% BG-11 – Cm: 40mL Plating: 1% BG-11 – no resistance -20hrà Cm C: Liquid: BG-11 – no resistance, 50:150 C: Liquid: BG-11 – Cm: 50:150 Liquid: BG-11 – no resistance 50:150 -20hrà Cm
- Inoculated mevT, pBAD24, PSB1A3
- prep of more PSB1A3
- gel extraction of pBAD24, PSB1A3, GsSDMT, and PCR GsSDMT
- double digest of the above with appropriate enzymes
- ligation of the biobrick attempt
- cyano transformation attempt
