From 2009.igem.org
Calendar
August 4, 2009
Cyanos: Transformations à 30°C room with Parafilm
1. Low 1 – Culture started on 7/27/09 - 23 mg/ml
2. Low 2 - Culture started on 7/27/09 – 32 mg/ml
3. High 1 - Culture started on 7/20/09 – 23 mg/ml
4. High 2 - Culture started on 7/20/09 – 23 mg/ml
- Liquid cultures of High 1 and Low 1 were placed in 30°C Shaker, minimal indirect light
- Plated Cultures of High 1 and Low 1 were placed in minimal indirect light
C: Plating: 1% BG-11 – no resistance: 40mL
C: Plating: 1% BG-11 – Cm: 40mL
Plating: 1% BG-11 – no resistance -20hrà Cm
C: Liquid: BG-11 – no resistance, 50:150
C: Liquid: BG-11 – Cm: 50:150
Liquid: BG-11 – no resistance 50:150 -20hrà Cm
- GsSDMT Cloning
- Inoculated mevT, pBAD24, PSB1A3
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