From 2009.igem.org
Monday 10 August 2009
Results
Maria and Afton
- Transformation was successful. Results:
| Name | Plate | Growth Observation
|
| I1352 w/ pSB1C3
| Amp | ~100 colonies, 2 flourece pink
|
| I1352 w/ pSB1C3
| Cm | 5 colonies, 2 flourese pink
|
| I1352 w/ I1352
| Amp | all colonies are in one area, no florescence
|
Trentay 11:31, 10 August 2009 (UTC)
Tasks
- 1 Today parts were digesting and ligating into full plasmids then transformed into E. coli NEB10β. UP element D1 was ligated onto constitutive promoter BBa_J23101 on plasmid pSB1C3. UP element with promoters D2 - D9 were ligated to RET BBa_J06702 on pSB1C3.
Bussingkm 13:45, 10 August 2009 (UTC)
Wetlab
- 1 Wetlab work went relatively smoothly. E. coli transformations occurred at roughly 6:00 PM. During digestion, I did not start the hot water bath soon enough but gel electrophoresis showed the correct cutting before ligation. Procedure was followed well. Bussingkm 20:09, 11 August 2009 (UTC)
- 2
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