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Virginia Commonwealth/25 June 2009
From 2009.igem.org
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Contents |
Thursday 25 June 2009
Results
- At 9:30 am the plate with the e.coli transformed with part pSB3K3 on LB+KAN grew approximately 50 small colonies.
- The plate growing the pSB3K3+BBa_J23102+E0240 assembly on LB+KAN grew approximately 50 small colonies. Neither growth expressed fluorescence.
Tasks
- 1 Promoter Design Meeting
- we discussed different methods of promoter design.
- Using a paper that charted the % variance in the -10/-35 sigma binding region, we will design 16 promooters by changing the column with the smallest variances. Promoters will be documented upon completion.
- After designing 16 promoters, we will change multiple columns at the same time, creating several more promoters to test.
- Additional design and testing ideas include:
- Creating and changing an 'up element' and testing how it changes the strength (more reading is necessary since this is a field of uncharted waters).
- Adding the up element to our promoters and find the bonding strength of the sigma factors (sigma 70, sigma 38, and sigma 34).
- Adding positive and negative controls to each set up and test for strength and if anything was altered or affected in any way by the addition. A design tree for this idea can be found on page 14 of the lab notebook.
Wetlab
- Colonies from the pSB3K3 plate were picked and grown overnight in LB+KAN culture.
