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Virginia Commonwealth/30 May 2009
From 2009.igem.org
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==Saturday 30 May 2009== | ==Saturday 30 May 2009== | ||
===Results=== | ===Results=== | ||
| + | # 15% glycerol stocks stored in -80, box C15. | ||
| + | # Miniprep completed as in [[http://openwetware.org/wiki/Miniprep/Qiagen_kit|the OWW protocol]], doing two elutions of 20uL and 30uL to increase yield. Spec. used to assess yields: | ||
| + | {| cellpadding="10" align="center" border="0.9" padding="5" | ||
| + | |+ | ||
| + | |- | ||
| + | ! Sample!! A260 !! A280 !! Ratio !! Conc. (ug/ml) !! Amount (ug) | ||
| + | |- | ||
| + | ! J04450_1 | ||
| + | | 0.082 || 0.050 || 1.640 ||123 || ~5.6 | ||
| + | |- | ||
| + | ! J04450_2 | ||
| + | | 0.077 || 0.047 || 1.638 || 115 || ~5.3 | ||
| + | |- | ||
| + | ! I13521 | ||
| + | | 0.082 || 0.049 || 1.673 || 123 || ~5.6 | ||
| + | |} | ||
| + | # Plasmids were stored in -20 in box marked "George's stocks" | ||
===Tasks=== | ===Tasks=== | ||
# Glycerol stocks and miniprep of plasmids from overnight cultures of J04450 and I13521. | # Glycerol stocks and miniprep of plasmids from overnight cultures of J04450 and I13521. | ||
Revision as of 16:35, 30 May 2009
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Saturday 30 May 2009
Results
- 15% glycerol stocks stored in -80, box C15.
- Miniprep completed as in [OWW protocol], doing two elutions of 20uL and 30uL to increase yield. Spec. used to assess yields:
| Sample | A260 | A280 | Ratio | Conc. (ug/ml) | Amount (ug) |
|---|---|---|---|---|---|
| J04450_1 | 0.082 | 0.050 | 1.640 | 123 | ~5.6 |
| J04450_2 | 0.077 | 0.047 | 1.638 | 115 | ~5.3 |
| I13521 | 0.082 | 0.049 | 1.673 | 123 | ~5.6 |
- Plasmids were stored in -20 in box marked "George's stocks"
Tasks
- Glycerol stocks and miniprep of plasmids from overnight cultures of J04450 and I13521.
