Wisconsin-Madison/16 July 2009

From 2009.igem.org

Calendar

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31

July 16, 2009

Other:

Advisor Meeting (only Jenny)

Faxing

Research

Waiting for Primers


Ex 22: Triple Induction

Growth: Transformation of Double - Triple: good

(pic here)

Inoculate:

1. Triple: DH1 – Terrific Broth

2. Triple: DH1 – M9 Media


Double Digestion of BB (X+P)

1. NudF (560+2157=) 2717 bp

2. Choline (2000+2157=) 4157 bp

3. BB (2157+1069=) 3226 bp control

F F1 F2 C

0716 gel.jpg 0710 ladder.jpg


Ex 18: Taq

Chris ran PCR with taq protein – YhfR

If Fails, Taq fault, we have YhfR as BioBrick

Retrieved from "http://2009.igem.org/Wisconsin-Madison/16_July_2009"