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Virginia Commonwealth/13 June 2009
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==Saturday 13 June 2009== | ==Saturday 13 June 2009== | ||
===Results=== | ===Results=== | ||
| - | * | + | *cultivations were successful |
---- | ---- | ||
===Tasks=== | ===Tasks=== | ||
---- | ---- | ||
| - | * | + | *mini-preps on backbone and reporter |
| - | * | + | *freeze cells for storage |
====Wetlab==== | ====Wetlab==== | ||
Mini-Prep results | Mini-Prep results | ||
Revision as of 01:59, 19 June 2009
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Contents |
Saturday 13 June 2009
Results
- cultivations were successful
Tasks
- mini-preps on backbone and reporter
- freeze cells for storage
Wetlab
Mini-Prep results
| Part # | A260 | A260/A280 | A280 | Volume (uL) | Conc. (ug/mL) | Amt. (ug) |
| pSB3K3 (1) | .016 | 1.231 | .013 | 41 | 24 | .984 |
| pSB3K3 (2) | .020 | 1.111 | .018 | 41.2 | 30 | 1.236 |
| pSB3K3 (3) | .021 | 1.167 | .018 | 41.6 | 31.5 | 1.310 |
| pSB3K3 (4) | .030 | 1.154 | .026 | 43.0 | 45 | 1.935 |
| E0240 (1) | .020 | 1.217 | .023 | 40.0 | 30. | 1.200 |
| E0240 (2) | .025 | 1.190 | .021 | 42.0 | 37.5 | 1.575 |
| E0240 (3) | .030 | 1.200 | .025 | 41.5 | 45. | 1.868 |
| E0240 (4) | .033 | 1.269 | .026 | 39.0 | 49.5 | 1.931 |
- Samples were diluted 30x for spectrophotometry. A260=50 (ug/mL)
- Cell stock was frozen and stored in box C-15.
- DNA was stored in the lab freezer.
-Bussingkm 15:52, 13 June 2009 (UTC)
