From 2009.igem.org
Saturday 13 June 2009
Results
- cultivations were successful
Tasks
- mini-preps on backbone and reporter
- freeze cells for storage
Wetlab
Mini-Prep results
Part # | A260 | A260/A280 | A280 | Volume (uL) | Conc. (ug/mL) | Amt. (ug)
|
pSB3K3 (1) | .016 | 1.231 | .013 | 41 | 24 | .984
|
pSB3K3 (2) | .020 | 1.111 | .018 | 41.2 | 30 | 1.236
|
pSB3K3 (3) | .021 | 1.167 | .018 | 41.6 | 31.5 | 1.310
|
pSB3K3 (4) | .030 | 1.154 | .026 | 43.0 | 45 | 1.935
|
E0240 (1) | .020 | 1.217 | .023 | 40.0 | 30. | 1.200
|
E0240 (2) | .025 | 1.190 | .021 | 42.0 | 37.5 | 1.575
|
E0240 (3) | .030 | 1.200 | .025 | 41.5 | 45. | 1.868
|
E0240 (4) | .033 | 1.269 | .026 | 39.0 | 49.5 | 1.931
|
- Samples were diluted 30x for spectrophotometry. A260=50 (ug/mL)
- Cell stock was frozen and stored in box C-15.
- DNA was stored in the lab freezer.
-Bussingkm 15:52, 13 June 2009 (UTC)
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