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Virginia Commonwealth/18 August 2009
From 2009.igem.org
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==Tuesday 18 August 2009== | ==Tuesday 18 August 2009== | ||
===Results=== | ===Results=== | ||
| - | * | + | ''Maria and Afton'' |
| - | * | + | * Re-Inoculated cultures with 10µ sample in 5mL of LB+Amp show no expression |
| + | ** Overnight cultures made Sunday: only J23116 is expressing florescence (slightly pink) | ||
| + | * Electrocompetency was completed | ||
| + | ** 80 vials of 250µL Electrocompetent NEB10β was made | ||
| + | ** They will be tested today to ensure the procedure was successful | ||
| + | [[User:Trentay|Trentay]] 14:11, 18 August 2009 (UTC) | ||
---- | ---- | ||
| + | |||
===Tasks=== | ===Tasks=== | ||
| - | + | ''Kevin'' | |
| - | * | + | * PCR |
| + | |||
| + | ''Maria and Afton'' | ||
| + | * Do Spectrophotometry of E0240 and pSB1C3 | ||
| + | * Digest E0240 and pSB1C3 w/p1010 | ||
| + | * Run a gel on E0240 and pSB1C3 w/p1010 | ||
| + | * Ligate E0240 w/ pSB1C3 with J23100-104 | ||
| + | ** Digests from last week will be used for ligation | ||
| + | ** However, I would prefer to transform Ligations with J06702 and see how well those grow before proceeding to further ligations | ||
| + | |||
| + | * Transform Ligations with pSB1C3 and J06702 with: J23100-J23107, R0011, R0040 | ||
| + | * Transform newly made electrocompetent cells to make sure they are working | ||
| + | [[User:Trentay|Trentay]] 14:20, 18 August 2009 (UTC) | ||
---- | ---- | ||
====Wetlab==== | ====Wetlab==== | ||
| + | ''Maria and Afton'' | ||
* 1 | * 1 | ||
* 2 | * 2 | ||
| + | [[User:Trentay|Trentay]] 14:20, 18 August 2009 (UTC) | ||
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Contents |
Tuesday 18 August 2009
Results
Maria and Afton
- Re-Inoculated cultures with 10µ sample in 5mL of LB+Amp show no expression
- Overnight cultures made Sunday: only J23116 is expressing florescence (slightly pink)
- Electrocompetency was completed
- 80 vials of 250µL Electrocompetent NEB10β was made
- They will be tested today to ensure the procedure was successful
Trentay 14:11, 18 August 2009 (UTC)
Tasks
Kevin
- PCR
Maria and Afton
- Do Spectrophotometry of E0240 and pSB1C3
- Digest E0240 and pSB1C3 w/p1010
- Run a gel on E0240 and pSB1C3 w/p1010
- Ligate E0240 w/ pSB1C3 with J23100-104
- Digests from last week will be used for ligation
- However, I would prefer to transform Ligations with J06702 and see how well those grow before proceeding to further ligations
- Transform Ligations with pSB1C3 and J06702 with: J23100-J23107, R0011, R0040
- Transform newly made electrocompetent cells to make sure they are working
Trentay 14:20, 18 August 2009 (UTC)
Wetlab
Maria and Afton
- 1
- 2
Trentay 14:20, 18 August 2009 (UTC)
