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Virginia Commonwealth/21 May 2009
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##BBa_J23101 (Promoter) - located in plate 1, well 18E ''AmpR'' | ##BBa_J23101 (Promoter) - located in plate 1, well 18E ''AmpR'' | ||
##BBa_I20260 (Standard promoter) - located in plate 2, well 17F ''KanR'' | ##BBa_I20260 (Standard promoter) - located in plate 2, well 17F ''KanR'' | ||
| - | #Plate cells on LB with appropriate antibiotic | + | #Plate cells on LB with appropriate antibiotic - grow overnight |
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===Results=== | ===Results=== | ||
#Plates were prepared for today's work. | #Plates were prepared for today's work. | ||
Revision as of 21:35, 21 May 2009
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Thursday 21 May 2009
Tasks
- Make stock of electrocompetent E. coli K-12
- Transform E. coli with the following:
- BBa_I13521 (RPF PRET) - located in plate 2, well 6O AmpR
- BBa_I13522 (GRP PRET) located in plate 2, well 8A AmpR
- BBa_E0840 (GFP RET) - located in plate 1, well 12O AmpR
- BBa_R0040 (Promoter) - located in plate 1, well 6I AmpR
- pSB1AK3-J04450 (KanR plasmid w/ RPF insert) - located in plate 1, well 13A KanR
- BBa_E0240 (GFP RET) - located in plate 1, well 12M AmpR
- BBa_J23101 (Promoter) - located in plate 1, well 18E AmpR
- BBa_I20260 (Standard promoter) - located in plate 2, well 17F KanR
- Plate cells on LB with appropriate antibiotic - grow overnight
Results
- Plates were prepared for today's work.
