Virginia Commonwealth/4 June 2009

From 2009.igem.org

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===Tasks===
===Tasks===
#Transform NEB10beta with the following parts:
#Transform NEB10beta with the following parts:
-
#*R0040
+
#*[http://partsregistry.org/Part:BBa_R0040 R0040] (Promoter)
-
#*E0840
+
#*[http://partsregistry.org/Part:BBa_E0840 E0840] (GFP RET)
-
#*J13002
+
#*[http://partsregistry.org/Part:BBa_J13002 J13002] (Expression plasmid) ''AmpR''
-
#*E1010
+
#*[http://partsregistry.org/Part:BBa_E1010 E1010] (RFP CDS) ''KanR''
-
#*pSB1C3
+
#*[http://partsregistry.org/wiki/index.php?title=Part:pSB1C3 pSB1C3] (High-copy assembly plasmid) ''CmR''
-
#*pSB4C5
+
#*[http://partsregistry.org/wiki/index.php?title=Part:pSB4C5 pSB4C5] (Low-copy assembly plasmid) ''CmR''
#Run gel of single and double digests on I13521
#Run gel of single and double digests on I13521

Revision as of 13:18, 4 June 2009

May
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Thursday 04 June 2009

Results

Tasks

  1. Transform NEB10beta with the following parts:
    • R0040 (Promoter)
    • E0840 (GFP RET)
    • J13002 (Expression plasmid) AmpR
    • E1010 (RFP CDS) KanR
    • pSB1C3 (High-copy assembly plasmid) CmR
    • pSB4C5 (Low-copy assembly plasmid) CmR
  2. Run gel of single and double digests on I13521