Virginia Commonwealth/4 June 2009

From 2009.igem.org

(Difference between revisions)
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===Tasks===
===Tasks===
#Transform NEB10beta with the following parts:
#Transform NEB10beta with the following parts:
-
#*[http://partsregistry.org/Part:BBa_R0040 R0040] (Promoter) ''AmpR''
+
{| cellpadding="10" align="center" border="0.9" padding="5"
-
#*[http://partsregistry.org/Part:BBa_E0840 E0840] (GFP RET) ''AmpR''
+
|+
-
#*[http://partsregistry.org/Part:BBa_J13002 J13002] (Expression plasmid) ''AmpR''
+
|-
-
#*[http://partsregistry.org/Part:BBa_E1010 E1010] (RFP CDS) ''KanR''
+
! Part No.!! Type !! Resistance !! Plate !! Well !!
-
#*[http://partsregistry.org/wiki/index.php?title=Part:pSB1C3 pSB1C3] (High-copy assembly plasmid) ''CmR''
+
|-
-
#*[http://partsregistry.org/wiki/index.php?title=Part:pSB4C5 pSB4C5] (Low-copy assembly plasmid) ''CmR''
+
! [http://partsregistry.org/Part:BBa_R0040 R0040]  
-
#Run gel of single and double digests on I13521
+
| Promoter || AmpR || 1 || ? ||
 +
|-
 +
! [http://partsregistry.org/Part:BBa_E0840 E0840]
 +
| GFP RET || AmpR || 1 || 12O ||
 +
|-
 +
! [http://partsregistry.org/Part:BBa_J13002 J13002]
 +
| Expression plasmid || AmpR || ? || ? ||
 +
|-
 +
! [http://partsregistry.org/Part:BBa_E1010 E1010]  
 +
| RFP CDS || KanR || ? || ? ||
 +
|-
 +
! [http://partsregistry.org/wiki/index.php?title=Part:pSB1C3 pSB1C3]  
 +
| High-copy assembly plasmid || CmR || ? || ? ||
 +
|-
 +
! [http://partsregistry.org/wiki/index.php?title=Part:pSB4C5 pSB4C5]  
 +
| Low-copy assembly plasmid || CmR || ? ||? ||
 +
|}
 +
#Run gel of single and double digests on I13521 (George and Chris)

Revision as of 13:36, 4 June 2009

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Thursday 04 June 2009

Results

Tasks

  1. Transform NEB10beta with the following parts:
Part No. Type Resistance Plate Well
R0040 Promoter AmpR 1  ?
E0840 GFP RET AmpR 1 12O
J13002 Expression plasmid AmpR  ?  ?
E1010 RFP CDS KanR  ?  ?
pSB1C3 High-copy assembly plasmid CmR  ?  ?
pSB4C5 Low-copy assembly plasmid CmR  ? ?
  1. Run gel of single and double digests on I13521 (George and Chris)
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