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Virginia Commonwealth/4 June 2009
From 2009.igem.org
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===Results=== | ===Results=== | ||
===Tasks=== | ===Tasks=== | ||
| - | + | *Transform NEB10beta with the following parts: | |
{| cellpadding="10" align="center" border="0.9" padding="5" | {| cellpadding="10" align="center" border="0.9" padding="5" | ||
|+ | |+ | ||
|- | |- | ||
| - | ! Part No.!! Type !! Resistance !! Plate !! Well !! | + | ! Part No.!! Type !! ccdB gene !! Resistance !! Plate !! Well !! |
|- | |- | ||
! [http://partsregistry.org/Part:BBa_R0040 R0040] | ! [http://partsregistry.org/Part:BBa_R0040 R0040] | ||
| - | | Promoter || AmpR || N/A || N/A || | + | | Promoter || No || AmpR || N/A || N/A || |
|- | |- | ||
! [http://partsregistry.org/Part:BBa_E0840 E0840] | ! [http://partsregistry.org/Part:BBa_E0840 E0840] | ||
| - | | GFP RET || AmpR || N/A || N/A || | + | | GFP RET || No || AmpR || N/A || N/A || |
|- | |- | ||
! [http://partsregistry.org/Part:BBa_J13002 J13002] | ! [http://partsregistry.org/Part:BBa_J13002 J13002] | ||
| - | | Expression plasmid || AmpR || | + | | Expression plasmid || No || AmpR || 1 || 13B || |
|- | |- | ||
! [http://partsregistry.org/Part:BBa_E1010 E1010] | ! [http://partsregistry.org/Part:BBa_E1010 E1010] | ||
| - | | RFP CDS || KanR || | + | | RFP CDS || No || KanR || 1 || 18F || |
|- | |- | ||
! [http://partsregistry.org/wiki/index.php?title=Part:pSB1C3 pSB1C3] | ! [http://partsregistry.org/wiki/index.php?title=Part:pSB1C3 pSB1C3] | ||
| - | | High-copy assembly plasmid || CmR || | + | | High-copy assembly plasmid || No || CmR || 1 || 5A || |
|- | |- | ||
! [http://partsregistry.org/wiki/index.php?title=Part:pSB4C5 pSB4C5] | ! [http://partsregistry.org/wiki/index.php?title=Part:pSB4C5 pSB4C5] | ||
| - | | Low-copy assembly plasmid || CmR || | + | | Low-copy assembly plasmid || Yes || CmR || 1 ||5I || |
|} | |} | ||
| - | + | *Run gel of single and double digests on I13521 (George and Chris) | |
Revision as of 13:47, 4 June 2009
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Thursday 04 June 2009
Results
Tasks
- Transform NEB10beta with the following parts:
| Part No. | Type | ccdB gene | Resistance | Plate | Well | |
|---|---|---|---|---|---|---|
| R0040 | Promoter | No | AmpR | N/A | N/A | |
| E0840 | GFP RET | No | AmpR | N/A | N/A | |
| J13002 | Expression plasmid | No | AmpR | 1 | 13B | |
| E1010 | RFP CDS | No | KanR | 1 | 18F | |
| pSB1C3 | High-copy assembly plasmid | No | CmR | 1 | 5A | |
| pSB4C5 | Low-copy assembly plasmid | Yes | CmR | 1 | 5I |
- Run gel of single and double digests on I13521 (George and Chris)
