Virginia Commonwealth/4 June 2009
From 2009.igem.org
(Difference between revisions)
GMcArthurIV (Talk | contribs) |
GMcArthurIV (Talk | contribs) |
||
Line 40: | Line 40: | ||
===Results=== | ===Results=== | ||
===Tasks=== | ===Tasks=== | ||
+ | *Lab orientation and safety overview | ||
+ | *Synthetic biology overview discussion | ||
+ | *Literature discussion | ||
+ | ====Wetlab==== | ||
+ | *Introduction to media making | ||
*Transform NEB10β or DB3.1 with the following parts: | *Transform NEB10β or DB3.1 with the following parts: | ||
{| cellpadding="10" align="center" border="0.9" padding="5" | {| cellpadding="10" align="center" border="0.9" padding="5" |
Revision as of 15:01, 4 June 2009
|
|
|
|
|
|
Contents |
Thursday 04 June 2009
Results
Tasks
- Lab orientation and safety overview
- Synthetic biology overview discussion
- Literature discussion
Wetlab
- Introduction to media making
- Transform NEB10β or DB3.1 with the following parts:
Part No. | Type | ccdB gene | Resistance | Plate | Well | |
---|---|---|---|---|---|---|
R0040 | Promoter | No | AmpR | N/A | N/A | |
E0840 | GFP RET | No | AmpR | N/A | N/A | |
J13002 | Expression plasmid | No | AmpR | 1 | 13B | |
E1010 | RFP CDS | No | KanR | 1 | 18F | |
pSB1C3 | High-copy assembly plasmid | No | CmR | 1 | 5A | |
pSB4C5 | Low-copy assembly plasmid | Yes | CmR | 1 | 5I |
- Run gel of single and double digests on I13521 (George and Chris)