Virginia Commonwealth/4 June 2009
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==Thursday 04 June 2009== | ==Thursday 04 June 2009== | ||
===Results=== | ===Results=== | ||
+ | *Overnight 5mL culture of I13521 looks great (i.e., it's pinkish red) - [[User:GMcArthurIV|GMcArthurIV]] 15:03, 4 June 2009 (UTC) | ||
===Tasks=== | ===Tasks=== | ||
*Lab orientation and safety overview | *Lab orientation and safety overview |
Revision as of 15:03, 4 June 2009
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Contents |
Thursday 04 June 2009
Results
- Overnight 5mL culture of I13521 looks great (i.e., it's pinkish red) - GMcArthurIV 15:03, 4 June 2009 (UTC)
Tasks
- Lab orientation and safety overview
- Synthetic biology overview discussion
- Literature discussion
Wetlab
- Introduction to media making
- Transform NEB10β or DB3.1 with the following parts:
Part No. | Type | ccdB gene | Resistance | Plate | Well | |
---|---|---|---|---|---|---|
R0040 | Promoter | No | AmpR | N/A | N/A | |
E0840 | GFP RET | No | AmpR | N/A | N/A | |
J13002 | Expression plasmid | No | AmpR | 1 | 13B | |
E1010 | RFP CDS | No | KanR | 1 | 18F | |
pSB1C3 | High-copy assembly plasmid | No | CmR | 1 | 5A | |
pSB4C5 | Low-copy assembly plasmid | Yes | CmR | 1 | 5I |
- Run gel of single and double digests on I13521 (George and Chris)