Virginia Commonwealth/4 June 2009

From 2009.igem.org

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! [http://partsregistry.org/wiki/index.php?title=Part:pSB4C5 pSB4C5]  
! [http://partsregistry.org/wiki/index.php?title=Part:pSB4C5 pSB4C5]  
-
| Low-copy assembly plasmid || Yes || CmR || 1 ||5I ||
+
| Low-copy assembly plasmid || Yes || CmR || 1 ||5I ||
 +
|}
 +
*Controls (electroporate NEB10β or DB3.1 without plasmid DNA and grow on agar overnight):
 +
{| cellpadding="10" align="center" border="0.9" padding="5"
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|+
 +
|-
 +
! Part No.!! Type !! Resistant to ccdB !! Resistance !!
 +
|-
 +
! (+) Control
 +
| NEB10β on LB || No || None ||
 +
|-
 +
! (-) Control
 +
| NEB10β on LB+Amp || No || None ||
 +
|-
 +
! (-) Control
 +
| NEB10β on LB+Cm || No || None ||
 +
|-
 +
! (-) Control
 +
| NEB10β on LB+Kan || No || None ||
 +
|-
 +
! (-) Control
 +
| DB3.1 on LB+Cm || Yes || None ||
|-
|-
-
! Control  
+
! (+) Control  
-
| NEB10β on LB || - || None || - ||- ||  
+
| DB3.1 on LB+Kan || Yes || None ||
|}
|}
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*controls
 
-
*NEB10β (+) LB
 
-
*NEB10β (-) AMP
 
-
*NEB10β (-) CM
 
-
*NEB10β (-) KAN
 
-
*DB3.1 (-) CM
 
-
*DB3.1 (+) LB
 

Revision as of 23:22, 5 June 2009

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Thursday 04 June 2009

Results

  • Overnight 5mL culture of I13521 looks great (i.e., it's pinkish red) - GMcArthurIV 15:03, 4 June 2009 (UTC)

Tasks

  • Lab orientation and safety overview
  • Synthetic biology overview discussion

Wetlab

  • Transform NEB10β or DB3.1 with the following parts and plate overnight:
Part No. Type ccdB gene Resistance Plate Well
R0040 Promoter No AmpR N/A N/A
E0840 GFP RET No AmpR N/A N/A
J13002 Expression plasmid No AmpR 1 13B
E1010 RFP CDS No KanR 1 18F
pSB1C3 High-copy assembly plasmid No CmR 1 5A
pSB4C5 Low-copy assembly plasmid Yes CmR 1 5I
  • Controls (electroporate NEB10β or DB3.1 without plasmid DNA and grow on agar overnight):
Part No. Type Resistant to ccdB Resistance
(+) Control NEB10β on LB No None
(-) Control NEB10β on LB+Amp No None
(-) Control NEB10β on LB+Cm No None
(-) Control NEB10β on LB+Kan No None
(-) Control DB3.1 on LB+Cm Yes None
(+) Control DB3.1 on LB+Kan Yes None
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