Virginia Commonwealth/5 June 2009

From 2009.igem.org

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===Results===
===Results===
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*Observations on the Colonies
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{| cellpadding="10" align="center" border="0.9" padding="5"
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|+
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|-
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! Part No.!! Plate Type !! Colonies !! Other Observations !!
 +
|-
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! J13002
 +
| Amp || 10s of colonies || n/a ||
 +
|-
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! PSB 1C3
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| Cm || 10s of colonies || The colonies are pinkish in color because the rfp is being expressed. ||
 +
|-
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! R0040
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| Amp || 10s of colonies || n/a ||
 +
|-
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! E1010
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| Kan || 100s of colonies  || n/a ||
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|-
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! PSB 4C5
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| Cm || about 9 colonies || n/a ||
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|-
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! E0840
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| Amp ||10s of colonies || n/a ||
 +
|}
 +
 +
*Observations on the Controls
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 +
{| cellpadding="10" align="center" border="0.9" padding="5"
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|+
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|-
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! Control !! Plate Type !! Observation !!
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|-
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! NEB10β (+)
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| LB || lawn ||
 +
|-
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! NEB10β (-)
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| Cm || no growth ||
 +
|-
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! NEB10β (-)
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| Amp || no growth ||
 +
|-
 +
! NEB10β (-)
 +
| Kan || no growth ||
 +
|-
 +
! DB3.1 (-)
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| Cm || no growth ||
 +
|-
 +
! DB3.1(+)
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| LB ||lawn ||
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|}
 +
 +
[[User:MandM|MandM]] 16:14, 7 June 2009 (UTC)
 +
===Tasks===
===Tasks===
----
----

Revision as of 02:36, 11 June 2009

May
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June
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August
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October
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Contents

Friday 05 June 2009

Results

  • Observations on the Colonies
Part No. Plate Type Colonies Other Observations
J13002 Amp 10s of colonies n/a
PSB 1C3 Cm 10s of colonies The colonies are pinkish in color because the rfp is being expressed.
R0040 Amp 10s of colonies n/a
E1010 Kan 100s of colonies n/a
PSB 4C5 Cm about 9 colonies n/a
E0840 Amp 10s of colonies n/a
  • Observations on the Controls
Control Plate Type Observation
NEB10β (+) LB lawn
NEB10β (-) Cm no growth
NEB10β (-) Amp no growth
NEB10β (-) Kan no growth
DB3.1 (-) Cm no growth
DB3.1(+) LB lawn

MandM 16:14, 7 June 2009 (UTC)

Tasks

  • Literature discussion
  • Genetic engineering work flow discussion

Wetlab

  • Pick transformed colonies and culture overnight (5mL)
  • Introduction to media making
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