Virginia Commonwealth/6 June 2009

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Revision as of 16:32, 9 June 2009 by GMcArthurIV (Talk | contribs)
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Contents

Saturday 06 June 2009

Results

  • Note that undergraduates need key card access to the building.
  • All samples from yesterday's cultivation grew well.

Tasks


  • Harvested cells from cultivation
  • Purified plasmids using the mini-prep kit
  • Froze stock of cells carrying plasmids in box C-15

Wetlab

  • Mini-prep (plasmid purification) tutorial
    • Data from plasmid purification
Part No. A260 (nm) A280 (nm) A260/A280 Concentration (microgram/microliter) Amount (microgram) Volume (microliter)
J13002 .26 .018 1.44 52 1300 25
PSB 1C3 .085 .050 1.700 17 969 57
R0040 .025 .017 1.471 5 200 40
E1010 .063 .038 1.658 12.6 453 36
PSB 4C5 .101 .059 1.712 20.2 767 38
E0840 .084 .049 1.714 16.8 688 41

- Bussingkm 23:10, 6 June 2009 (UTC)